The assignment focuses on MALDI-TOF mass spectrometry – tools for bacterial identification. Also, compare and contrast the methods and give a critical analysis of the benefits and limitations of each method.
MALDI-TOF mass spectrometry – tools for bacterial identification
Firstly, you should discuss molecular methods (PCR) MALDI-TOF routine. Phenotypic culture methods in relation to the findings of the paper. The focus of the essay should include; speed of the assays turnaround times, sensitivity specificity of the methods technical training labour intensity cost implications. This assignment is NOT asking for a long, explanation of the science behind the methods. You asked to compare and contrast the methods and give a critical analysis of the benefits and limitations of each method. Also, Select one or two journal articles to support your discussion of molecular methods, NAATs. Remember, while MALDI TOF looks at molecules it is still a phenotypic method as it is looking at gene products (proteins). Cultures obtained on the day of collection invariably fail to demonstrate bacterially contaminated units that could develop clinically significant overgrowth during storage.
MALDI-TOF mass spectrometry – tools for bacterial identification
Also, the name of the journal article review has the following name. MALDI-TOF mass spectrometry tools for bacterial identification in clinical microbiology laboratory. Etienne Carbonnelle, Cécile Mesquita, Emmanuelle Bille, Nesrine Day, Brunhilde Dauphin, Jean-Luc Beretti. Agnès Ferroni, Laurent Gutmann, Xavier Nassif. Clinical Biochemistry 44 (2011) 104–109 I will upload it on additional materials. advantages and disadvantages of MALDI TOF versus molecular and phenotypic bacterial identification methods. Genetic probes generally have lower analytic sensitivity than other molecular assays due to the non amplified nature of the method. Therefore, the application is usually limited to specimens with high numbers of bacteria. Finally, streptococcal pharyngitis, positive blood cultures bottles, or isolate confirmation.
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