The assignment discusses Environmental Influences on Microbial Growth. Besides, there is also a description of Osmotic Pressure Assignment. So, Using the worksheet please convert the values for %T to O.D. in each column.
Environmental Influences on Microbial Growth-Osmotic Pressure Assignment
Bio 212 LAB – Environmental Influences on Microbial Growth, pH, Temp, and Osmotic Pressure. Assignment Instructions and Questions There are three chapters in this unit which are grouped together. Secondly, to elucidate how environmental or growing conditions can affect how microorganisms grow if their conditions are varied. Thirdly, these chapters are temperature, pH, and osmotic pressure effects on growth. Also, you will need to use the lab book for some background information and definitions as well as the worksheet provided (also posted in the Lab Notes on Canvas). You will be making three graphs for this assignment and then use them to answer questions.
Environmental Influences on Microbial Growth-Osmotic Pressure Assignment
Additionally, the data you will be graphing is derived from individual test tubes filled with microorganisms grown in different pH’s, Temps, etc. Each tube is measured on a Spectrophotometer and the % T is recorded. By doing this it is possible to see which organism grows best or least in the different conditions. Please refer to the posted Lab notes and your Lab book for an explanation on this. Answer all the questions for full credit, graphs alone are not sufficient. 1) Using the worksheet please convert the values for %T to O.D. in each column for the Temperature and pH data. You then will use the O.D. values only to make column graphs with the excel program.
Environmental Influences on Microbial Growth-Osmotic Pressure Assignment
Remember that O.D. = 2- log %T . So first take log %T ……..and then 2 minus that number for each. Once you convert these you can make the graphs on the excel program, which all computers will have. Note: on the worksheet “ Vis Read” means visual reading, referring to the turbidity of the tubes that we insert into the spectrophotometer to get the %T values.
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